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問下倒置生物顯微鏡如何辨別壞死細胞呢?

XINXIFENLEI:SHENGMINGKEXUEZIXUN  RIQI: 2011-12-5 13:18:53

分割線

問下倒置生物顯微鏡如何辨別壞死細胞呢?

上海光學儀器廠回復:
細胞壞死與細胞凋亡:細胞死亡可分細胞壞死和細胞凋亡兩種,其作用機制如下

〈1〉XIBAODIAOWANG〈apoptisis〉:DUOXIBAOSHENGWUZAIPEITAIFAZHANGUOCHENG、MIANYIDIAOJIEJIWEICHITINEIHUANJINGHENGDINGZHONG,BIXUJIEYOUYINFAZISHADE

JIZHILAIYICHUDUOYUHUOGONGNENGBUQUANDEXIBAO。ZHEZHONGJIZHIYINWEISHOUDAOYICHUANJIYINDEKONGZHI,GUYOUCHENGCHENGSHIXINGSIWANG〈programmed cell

death〉。ELIEDEHUANJINGRUGUOLENGHUOSHIGUORE、ZIWAIXIAN,QUEYANGHUOHUAXUEYAOWUDENGDOUKENENGSHIXIBAOSHANGHAI,QIDONGCHENGSHIYINFADIAOWANG,KEYI

BAOHUSHENGWUGETI,BIMIANXIBAOTUOLIKONGZHI,ZAOCHENGTUCHANG。XIBAODIAOWANGYIDANFASHENG,JIANGHUIZAOCHENGXIBAOHEXIAOSHI、DNAPIANDUANHUA、ZUIHOUXI

BG捕鱼BAOFENJIECHENGXUDUOJUYOUWANZHENGXIBAOMODEDIAOWANGXIAOTI〈cell bodies〉。YOUYUXIBAOMORENGWANZHENG,YINCIQINEISUOHANYOUDEXIBAOFENJIEHOUYOUDU

BG捕鱼WUZHIBINGBUHUISHIFANG,YINCIKEBIMIANZHOUZAOZUZHIDEFAYANHESHANGHAI。ZHEXIEDIAOWANGXIAOTIHENKUAIHUIBEIZHOUZAODETUNSHIXIBAO〈phagocytes〉HUOQI

TAXIBAOXISHOU。

BG捕鱼〈2〉XIBAOHUAISI〈necrosis〉:TONGCHANGSHIJIAOYANZHONGDEWULIHUAXUESHANGHAISUOZAOCHENG,ZHUYAOSHIXIBAOTONGTOUXINGFASHENGGAIBIANERPENGZHANGHUOPOLIE,BING

ZAOCHENGRONGTI〈lysosome 〉SHIFANGCHUGEZHONGFENJIEJIAOSUHEXIBAOJISU〈cytokines〉DENGWUZHI,ERSHIZHOUZAOZUZHISHOUSHANGYINQIFAYANFANYING。

3. SHUIZHONGWURANWUDUIRENLEIXIBAODEYINGXIANG:SHUIZHONGDEWURANWUZHONGLEIBAOHANWEISHENGWU,BAOKUOXIJUN、BINGDU、YUANSHENGDONGWUDENG;YOUJIWU,BAO

KUOGEZHONGHUIFAXINGYOUJIWU、HECHENGYOUJIWU、XIAODUFUCHANWU、QINGJIEJI、NONGYAODENG;WUJIWU,BAOKUOGEZHONGZHONGJINSHU、YANLEI、LV、RONGJIE

XINGGUTIWUDENG。ZHEXIEWURANWUDUIYURENTIDEYINGXIANGSHENJU,GUJIEYOUGUANCHAXIBAOJINGWUSHUICHULIHOUDEGAIBIAN,ZEKELEJIEZHEXIEWURANWUDUIYU

人類細胞的影響。
三. 材料

1. XIBAOZHU:RENLEIZIGONGJINGAIXIBAO〈HeLa cell〉

BG捕鱼2. SHIJI:ZHUILAN-YONGYURANXIBAO

              Saponin-JIEMIANHUOXINGJI,KEJIANGXIBAOMOJUNYUNDADONG,SHIMOJIEGOUSONGSAN

              H2O2-KEYINQICHENGSHIXINGSIWANG

             NaCl-PEIZHISHENGLISHIYANSHUI,0.9% NaClWEIRENDEDENGZHANGRONGYE

BG捕鱼3. DAICEYANGBEN:WAISHUANGXIXISHUI

4. 器材:載玻片、蓋玻片、微量吸管、光學顯微鏡


1.細胞鮮活良好時:在倒置生物顯微鏡下,可以看到細胞透亮,呈自然伸展狀態。狀態不好時,細胞部分伸展,或飄浮,壞死的細胞有點發黑并飄浮。

BG捕鱼2.XUANFUXIBAOZHUYAOSHIGENJUXIBAODETOULIANGCHENGDU,ZHUANGTAILIANGHAOSHI,XIBAOTOULIANG,ZHUANGTAIBUHAOSHI,KEJIANXIBAOYOUKELIZHUANG,FAHEI。

3.取部分細胞用4%的臺盼蘭染色,活細胞不著色,死細胞著色 
 

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