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酵母菌是真核细胞基因工程的基本研究工具

信息分类:生命科学资讯    作者:yiyi发布 

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JIAOMUJUNJIYINGONGCHENGJISHU(Saccharomyces cerevisiae Biotechnology)

JIAOMUJUNSHIZHENHEXIBAOJIYINGONGCHENGDEJIBENYANJIUGONGJU, YOURUE.ColiZHIYUYUANHEXIBAOYIBAN.

JIAOMUJUN(baker's yeast)ZAIPEIYANGYEZHONG, YUE90FENZHONGFENLIEYICI, QIEZAIYOUYANG, WUYANGHUANJINGJUNKECHENGZHANG.

JIAOMUJUNYOU16GEZHIXIANXINGRANSETI(DANTAO), ZUIXIAODE200Kb, ZUIDADE2,200Kb, QIEYOUGUANRANSETIFUZHIDEQISHIDIAN(Origins of replication) (ARS elements), ZHONGXINLI(centromeres, CEN elements)JIMODUANDUAN(telomeres)JUNYIBEIJIAJIECHULAI. QItelomeresHANYOU5dG-3dT3DEZHONGFUPIANDUAN.TONGSHIRENGONGRANSETIYEYOUCIBEIZHIZAOCHULAI, ERQIEYESHEJICHENGKEYIZAIE.ColiJIyeastZHONGJUNKECHENGZHANGDEshuttle vectors(BASHIJIAJIEMEIJIE).

JIAOMUJUNKEFENCHENGαHUOaXING, DANTAOJIAOMUJUNKEFENMIαHUOaDANBAIZHILAIYOUDAO"JIAOPEI"CHENGSHUANGTAO.

JIAOMUJUNYOUDEYEHANYOUZHITI, YOUDEYIHANYOUBINGDU.

JIAOMUJUNDECHENGZHANG, ZAIPEIYANGYEZHONGDADAO5×107cells/ml~2×108cells/mlWEIBAOHE, TONGCHANGYONGYPD(teast extract, peptone, dextrose)HUOCHENGYEPD medium.

YPD mediumPEIFANG:

MEIGONGSHENGHAN10g teast extract

20g peptone

20g dextrose

BAOCUNteast, KEYONGMEIZHIHAN1mlDE30﹪glycerol(XIAODUGUO)SHIGUAN, JIARU1ml yeastYE, BAOCUNZAI-70℃, HUO1ml yeastYEZHONG, JIARU80μlDEDMSO, BAOCUNZAI-70℃.

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